Isolation and Culture of Protoplasts from Luffa cylindrica Suspension Cultures
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چکیده
منابع مشابه
The isolation of plasma membrane from protoplasts of soybean suspension cultures.
A procedure for the isolation of plasma membranes from protoplasts of suspension-cultured soybean is described. Protoplasts were prepared by enzymic digestion of the cell wall and the plasma membrane was labelled with radioactive diazotized sulphanilic acid. The membrane systems from broken protoplasts were separated by continuous isopycnic sucrose gradient centrifugation. Radioactivity was loc...
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ژورنال
عنوان ژورنال: Plant tissue culture letters
سال: 1987
ISSN: 1884-7706,0289-5773
DOI: 10.5511/plantbiotechnology1984.4.35